Thin Film Biofabrication for Integrated Bio-electronics

This project integrates advances in biotechnology and microelectronics by developing generic, biologically-based thin film fabrication methods to incorporate biological function into electronics. Thin hydrogel films will be triggered to self-assemble in response to locally-imposed electrical signals. The PIs propose methods to incorporate small molecules, proteins, and cells into the hydrogel films, and will focus on understanding how fabrication conditions control the structure-property-function relations. A multilayer thin film system is proposed that will allow communication between the biology and the electronics. A collaboration with industrial partners will accelerate the incorporation of biology into materials science. The PIs also propose to integrate the activities within existing University partnerships with Food and Drug Administration and the National Institutes of Science and Technology. Because they provide the metrology and regulatory guidelines, these agencies will be integral to the speed that biologically-based materials are introduced into the marketplace. These partnerships will assist in accelerating translation and also in providing meaningful externships for students.

Publications

Parsed Synthesis of Pyocyanin via Co-culture Enables Context-dependent Intercellular Redox Communication

K. Chun, K. Stephens, S. Wang, C. Y. Tsao, G. F. Payne, and W. E. Bentley

Nov 2021

A Redox-Based Autoinduction Strategy to Facilitate Expression of 5xCys-Tagged Proteins for Electrobiofabrication

S. Wang, C. Y. Tsao, D. Motabar, J. Y. Li, G. F. Payne, and W. E. Bentley

Jun 2021

Bioelectronic Control of a Microbial Community using Surface-assembled Electrogenetic Cells to Route Signals

J. L. Terrell, T. Tschirhart, J. P. Jahnke, K. Stephens, Y. Liu, H. Dong, M. M. Hurley, M. Pozo, R. McKay, C. Y. Tsao, H. C. Wu, G. Vora, G. F. Payne, D. N. Stratis-Cullum, and W. E. Bentley

Mar 2021

3D-Printed Electrochemical Sensor-integrated Transwell Systems

P. R. Rajasekaran, A. A. Chapin, D. N. Quan, J. Herberholz, W. E. Bentley, and R. Ghodssi

Oct 2020

Electrochemical Measurement of Serotonin by Au-CNT Electrodes Fabricated on Microporous Cell Culture Membranes

A. A. Chapin, P. R. Rajasekaran, D. N. Quan, L. B. Hu, J. Herberholz, W. E. Bentley, and R. Ghodssi

Sep 2020

Homologous Quorum Sensing Regulatory Circuit: A Dual-Input Genetic Controller for Modulating Quorum Sensing-Mediated Protein Expression in E. coli

P. Hauk, K. Stephens, C. Virgile, E. VanArsdale, A. E. Pottash, J. S. Schardt, S. M. Jay, H. O. Sintim, and W. E. Bentley

Aug 2020

Transglutaminase-mediated Assembly of Multi-enzyme Pathway onto TMV Brush Surfaces for Synthesis of Bacterial Autoinducer-2

N. Bhokisham, Y. Liu, A. D. Brown, G. F. Payne, J. N. Culver, and W. E. Bentley

Jul 2020

Mediated Electrochemistry to Mimic Biology's Oxidative Assembly of Functional Matrices

J. Y. Li, E. Kim, K. M. Gray, C. Conrad, C. Y. Tsao, S. P. Wang, G. Zong, G. Scarcelli, K. M. Stroka, L. X. Wang, W. E. Bentley, and G. F. Payne

Jun 2020

A Redox-based Electrogenetic CRISPR System to Connect with and Control Biological Information Networks

N. Bhokisham, E. VanArsdale, K. T. Stephens, P. Hauk, G. F. Payne, and W. E. Bentley

May 2020

Synthetic Biology for Manipulating Quorum Sensing in Microbial Consortia

K. Stephens and W. E. Bentley

Apr 2020

Hierarchical Patterning via Dynamic Sacrificial Printing of Stimuli-responsive Hydrogels

H. J. Wen, J. Y. Li, G. F. Payne, Q. Feng, M. H. Liang, J. X. Chen, H. Dong, and X. D. Cao

Apr 2020

A Coculture Based Tyrosine-Tyrosinase Electrochemical Gene Circuit for Connecting Cellular Communication with Electronic Networks

E. VanArsdale, D. Hornstrom, G. Sjoberg, I. Jarbur, J. Pitzer, G. F. Payne, A. J. A. van Maris, and W. E. Bentley

Mar 2020

Quorum Sensing Communication: Molecularly Connecting Cells, Their Neighbors, and Even Devices in Annual Review of Chemical and Biomolecular Engineering, Vol 11

S. Wang, G. F. Payne, and W. E. Bentley

Mar 2020

Electrical Cuing of Chitosan's Mesoscale Organization

S. Wu, K. Yan, J. Y. Li, R. N. Huynh, C. B. Raub, J. N. Shen, X. W. Shi, and G. F. Payne

Mar 2020

Tag-Free Site-Specific BMP-2 Immobilization with Long-Acting Bioactivities via a Simple Sugar-Lectin Interaction

H. L. Wang, X. Qu, Z. Zhang, M. Lei, H. Q. Tan, C. Y. Bao, S. L. Lin, L. Y. Zhu, J. Kohn, and C. S. Liu

Feb 2020

Melanin Produced by the Fast-Growing Marine Bacterium Vibrio natriegens through Heterologous Biosynthesis: Characterization and Application

Z. Wang, T. Tschirhart, Z. Schultzhaus, E. E. Kelly, A. Chen, E. Oh, O. Nag, E. R. Glaser, E. Kim, P. F. Lloyd, P. T. Charles, W. Y. Li, D. Leary, J. Compton, D. A. Phillips, A. Dhinojwala, G. F. Payne, and G. J. Vora

Feb 2020

Multifunctional Conductive Hydrogel/Thermochromic Elastomer Hybrid Fibers with a Core-Shell Segmental Configuration for Wearable Strain and Temperature Sensors

J. X. Chen, H. J. Wen, G. L. Zhang, F. Lei, Q. Feng, Y. Liu, X. D. Cao, and H. Bong

Jan 2020

Redox-Channeling Polydopamine-Ferrocene (PDA-Fc) Coating To Confer Context-Dependent and Photothermal Antimicrobial Activities

J. L. Song, H. Liu, M. Lei, H. Q. Tan, Z. Y. Chen, A. Antoshin, G. F. Payne, X. Qu, and C. S. Liu

Jan 2020

Redox Activities of Melanins Investigated by Electrochemical Reverse Engineering: Implications for their Roles in Oxidative Stress

E. Kim, L. Panzella, A. Napolitano, and G. F. Payne

Dec 2019

Validation of Oxidative Stress Assay for Schizophrenia

E. Kim, Z. Keskey, M. Kang, C. Kitchen, W. E. Bentley, S. Chen, D. L. Kelly, and G. F. Payne

Oct 2019

Rapid Electroformation of Biopolymer Gels in Prescribed Shapes and Patterns: A Simpler Alternative to 3-D Printing

A. Gargava, S. Ahn, W. E. Bentley, and S. R. Raghavan

Sep 2019

Chip Modularity Enables Molecular Information Access from Organ-on-chip Devices with Quality Control

W. Shang, C. Y. Chen, K. Lo, G. F. Payne, and W. E. Bentley

Sep 2019

Bacterial Co-culture with Cell Signaling Translator and Growth Controller Modules for Autonomously Regulated Culture Composition

K. Stephens, M. Pozo, C. Y. Tsao, P. Hauk, and W. E. Bentley

Sep 2019

Pro- and Anti-oxidant Properties of Redox-Active Catechol-Chitosan Films

E. Kim, M. J. Kang, H. Liu, C. H. Cao, C. S. Liu, W. E. Bentley, X. Qu, and G. F. Payne

Jul 2019

Engineering Escherichia coli for Enhanced Sensitivity to the Autoinducer-2 Quorum Sensing Signal

K. Stephens, A. Zargar, M. Emamian, N. Abutaleb, E. Choi, D. N. Quan, G. Payne, and W. E. Bentley

Jul 2019

Redox Is a Global Biodevice Information Processing Modality

E. Kim, J. Y. Li, M. Kang, D. L. Kelly, S. Chen, A. Napolitano, L. Panzella, X. W. Shi, K. Yan, S. Wu, J. Shen, W. E. Bentley, and G. F. Payne

Apr 2019

Electrobiofabrication: Electrically Based Fabrication with Biologically Derived Materials

J. Y. Li, S. Wu, E. Kim, K. Yuan, H. Liu, C. S. Liu, H. Dong, X. Qu, X. W. Shi, J. N. Shen, W. E. Bentley, and G. F. Payne

Apr 2019

Role of Polydopamine's Redox-activity on its Pro-oxidant, Radical-scavenging, and Antimicrobial Activities

H. Liu, X. Qu, H. Q. Tan, J. L. Song, M. Lei, E. Kim, G. F. Payne, and C. S. Liu

Apr 2019

Redox-Based Synthetic Biology Enables Electrochemical Detection of the Herbicides Dicamba and Roundup via Rewired Escherichia coli

E. VanArsdale, C. Y. Tsao, Y. Liu, C. Y. Chen, G. F. Payne, and W. E. Bentley

Apr 2019

Programmable Electrofabrication of Porous Janus Films with Tunable Janus Balance for Anisotropic Cell Guidance and Tissue Regeneration

M. Lei, X. Qu, H. Liu, Y. Liu, S. J. Wang, S. Wu, W. E. Bentley, G. F. Payne, and C. S. Liu

Mar 2019

Electrochemical Reverse Engineering to Probe for Drug-phenol Redox Interactions

Y. T. Wang, Y. Liu, E. Kim, B. Li, and G. F. Payne

Feb 2019

Coupling Self-Assembly Mechanisms to Fabricate Molecularly and Electrically Responsive Films

J. Y. Li, D. Maniar, X. Qu, H. Liu, C. Y. Tsao, E. Kim, W. E. Bentley, C. S. Liu, and G. F. Payne

Jan 2019

Plasmid-encoded Protein Attenuates Escherichia coli Swimming Velocity and Cell Growth, not Reprogrammed Regulatory Functions

C. Virgile, P. Hauk, H. C. Wu, and W. E. Bentley

Jan 2019

An Immune Magnetic Nano-assembly for Specifically Amplifying Intercellular Quorum Sensing Signals

Y. G. Guan, C. Y. Tsao, D. N. Quan, Y. Li, L. Mei, J. L. Zhang, B. C. Zhang, Y. Liu, W. E. Bentley, G. F. Payne, and Q. Wang

Dec 2018

A Highly Conductive, Pliable and Foldable Cu/cellulose Paper Electrode Enabled by Controlled Deposition of Copper Nanoparticles

Y. Yang, Q. B. Huang, G. F. Payne, R. C. Sun, and X. H. Wang

Dec 2018

Selective Assembly and Functionalization of Miniaturized Redox Capacitor Inside Microdevices for Microbial Toxin and Mammalian Cell Cytotoxicity Analyses

W. Shang, Y. Liu, E. Kim, C. Y. Tsao, G. F. Payne, and W. E. Bentley

Oct 2018

Electrodeposition of a Magnetic and Redox-active Chitosan Film for Capturing and Sensing Metabolic Active Bacteria

Y. Li, Y. Liu, E. Kim, Y. Y. Song, C. Y. Tsao, Z. Teng, T. E. R. Gao, L. Mei, W. E. Bentley, G. F. Payne, and Q. Wang

Sep 2018

Catechol-chitosan Redox Capacitor for Added Amplification in Electrochemical Immunoanalysis

K. Yan, Y. Liu, Y. G. Guan, N. Bhokisham, C. Y. Tsao, E. Kim, X. W. Shi, Q. Wang, W. E. Bentley, and G. F. Payne

Sep 2018

Reverse Engineering To Characterize Redox Properties: Revealing Melanin's Redox Activity through Mediated Electrochemical Probing

M. Kang, E. Kim, Z. Temocin, J. Y. Li, E. Dadachova, Z. Wang, L. Panzella, A. Napolitano, W. E. Bentley, and G. F. Payne

Aug 2018

A Platform of Genetically Engineered Bacteria as Vehicles for Localized Delivery of Therapeutics: Toward Applications for Crohn's disease

R. McKay, M. Ghodasra, J. Schardt, D. Quan, A. E. Pottash, W. Shang, S. M. Jay, G. F. Payne, M. W. Chang, J. C. March, and W. E. Bentley

Aug 2018

Electrical Writing onto a Dynamically Responsive Polysaccharide Medium: Patterning Structure and Function into a Reconfigurable Medium

S. Wu, K. Yan, Y. A. Zhao, C. C. Tsai, J. Shen, W. E. Bentley, Y. Chen, H. B. Deng, Y. M. Du, G. F. Payne, and X. W. Shi

Aug 2018

Signal Processing Approach to Probe Chemical Space for Discriminating Redox Signatures

M. Kang, E. Kim, S. Chen, W. E. Bentley, D. L. Kelly, and G. F. Payne

Jul 2018

Radical Scavenging Activities of Biomimetic Catechol-Chitosan Films

C. H. Cao, E. Y. Kim, Y. Liu, M. Kang, J. Y. Li, J. J. Yin, H. Liu, X. Qu, C. S. Liu, W. E. Bentley, and G. F. Payne

Jun 2018

Electrofabrication of Functional Materials: Chloramine-based Antimicrobial Film for Infectious Wound Treatment

X. Qu, H. Liu, C. C. Zhang, Y. Lei, M. Lei, M. Xu, D. W. Jin, P. Li, M. Yin, G. F. Payne, and C. S. Liu

Jun 2018

Focusing Quorum Sensing Signalling by Nano-magnetic Assembly

Y. G. Guan, C. Y. Tsao, D. N. Quan, Y. Li, L. Mei, Y. Y. Song, B. C. Zhang, Y. Liu, G. F. Payne, W. E. Bentley, and Q. Wang

May 2018

Biofabricating Functional Soft Matter Using Protein Engineering to Enable Enzymatic Assembly

Y. Liu, H. C. Wu, N. Bhokisham, J. Y. Li, K. L. Hong, D. N. Quan, C. Y. Tsao, W. E. Bentley, and G. F. Payne

May 2018

Bio-inspired Redox-cycling Antimicrobial Film for Sustained Generation of Reactive Oxygen Species

H. Liu, X. Qu, E. Kim, M. Lei, K. Dai, X. L. Tan, M. Xu, J. Y. Li, Y. P. Liu, X. W. Shi, P. Li, G. F. Payne, and C. S. Liu

Apr 2018

Electrical Programming of Soft Matter: Using Temporally Varying Electrical Inputs To Spatially Control Self Assembly

K. Yan, Y. Liu, J. T. Zhang, S. O. Correa, W. Shang, C. C. Tsai, W. E. Bentley, J. Shen, G. Scarcelli, C. B. Raub, X. W. Shi, and G. F. Payne

Dec 2017

Connecting Biology to Electronics: Molecular Communication via Redox Modality

Y. Liu, J. Y. Li, T. Tschirhart, J. L. Terrell, E. Kim, C. Y. Tsao, D. L. Kelly, W. E. Bentley, and G. F. Payne

Oct 2017

Spectroelectrochemical Reverse Engineering Demonstrates That Melanin's Redox and Radical Scavenging Activities Are Linked

E. Kim, M. Kang, T. Tschirhart, M. Malo, E. Dadachova, G. Cao, J. J. Yin, W. E. Bentley, Z. Wang, and G. F. Payne

Oct 2017

The Analgesic Acetaminophen and the Antipsychotic Clozapine Can Each Redox-Cycle with Melanin

Z. Temocin, E. Kim, J. Y. Li, L. Panzella, M. L. Alfieri, A. Napolitano, D. L. Kelly, W. E. Bentley, and G. F. Payne

Sep 2017

Reliable Clinical Serum Analysis with Reusable Electrochemical Sensor: Toward Point-of-care Measurement of the Antipsychotic Medication Clozapine

M. Kang, E. Kim, T. E. Winkler, G. Banis, Y. Liu, C. A. Kitchen, D. L. Kelly, R. Ghodssi, and G. F. Payne

Sep 2017

Incorporating LsrK AI-2 Quorum Quenching Capability in a Functionalized Biopolymer Capsule

M. K. Rhoads, P. Hauk, J. Terrell, C. Y. Tsao, H. Oh, S. R. Raghavan, S. S. Mansy, G. F. Payne, and W. E. Bentley

Aug 2017

A Simple and Reusable Bilayer Membrane-based Microfluidic Device for the Study of Gradient-mediated Bacterial Behaviors

W. Shang, C. Y. Tsao, X. L. Luo, M. Teodoro, R. McKay, D. N. Quan, H. C. Wu, G. F. Payne, and W. E. Bentley

Aug 2017

Controlling Localization of Escherichia coli Populations using a Two-part Synthetic Motility Circuit: An Accelerator and Brake

R. McKay, P. Hauk, H. C. Wu, A. E. Pottash, W. Shang, J. Terrell, G. F. Payne, and W. E. Bentley

Jul 2017

Toward Understanding the Environmental Control of Hydrogel Film Properties: How Salt Modulates the Flexibility of Chitosan Chains

C.-C. Tsai, B. H. Morrow, W. Chen, G. F. Payne, and J. Shen

Jul 2017

Electrochemical Reverse Engineering: A Systems-level Tool to Probe the Redox-based Molecular Communication of Biology

J. Y. Li, Y. Liu, E. Kim, J. C. March, W. E. Bentley, and G. F. Payne

Apr 2017

Electrochemistry for Bio-device Molecular Communication: The Potential to Characterize, Analyze and Actuate Biological Systems

Y. Liu, E. Kim, J. Y. Li, M. J. Kang, W. E. Bentley, and G. F. Payne

Mar 2017

Mathematical model of LsrR- binding and derepression in Escherichia coli K12

S. M. Graff and W. E. Bentley

Feb 2017

Reversible Programing of Soft Matter with Reconfigurable Mechanical Properties

H. M. He, X. D. Cao, H. Dong, T. Ma, and G. F. Payne

Feb 2017

Redox Probing for Chemical Information of Oxidative Stress

E. Kim, T. E. Winkler, C. Kitchen, M. Kang, G. Banis, W. E. Bentley, D. L. Kelly, R. Ghodssi, and G. F. Payne

Dec 2016

Using a Redox Modality to Connect Synthetic Biology to Electronics: Hydrogel-Based Chemo-Electro Signal Transduction for Molecular Communication

Y. Liu, C.-Y. Tsao, E. Kim, T. Tschirhart, J. L. Terrell, W. E. Bentley, and G. F. Payne

Nov 2016

A Facile Two-Step Enzymatic Approach for Conjugating Proteins to Polysaccharide Chitosan at an Electrode Interface

N. Bhokisham, Y. Liu, H. Pakhchanian, G. F. Payne, and W. E. Bentley

Nov 2016

Fusing Sensor Paradigms to Acquire Chemical Information: An Integrative Role for Smart Biopolymeric Hydrogels

E. Kim, Y. Liu, H. Ben-Yoav, T. E. Winkler, K. Yan, X. W. Shi, J. Shen, D. L. Kelly, R. Ghodssi, W. E. Bentley, and G. F. Payne

Sep 2016

Conferring Biological Activity to Native Spider Silk: A Biofunctionalized Protein-Based Microfiber

H. C. Wu, D. N. Quan, C. Y. Tsao, Y. Liu, J. L. Terrell, X. L. Luo, J. C. Yang, G. F. Payne, and W. E. Bentley

Aug 2016

Electrochemical Probing through a Redox Capacitor To Acquire Chemical Information on Biothiols

Z. C. Liu, Y. Liu, E. Kim, W. E. Bentley, and G. F. Payne

Jul 2016

Paraquat-Melanin Redox-Cycling: Evidence from Electrochemical Reverse Engineering

E. Kim, W. T. Leverage, Y. Liu, L. Panzella, M. L. Alfieri, A. Napolitano, W. E. Bentley, and G. F. Payne

Jun 2016

Probing Energy and Electron Transfer Mechanisms in Fluorescence Quenching of Biomass Carbon Quantum Dots

Z. C. Liang, M. J. Kang, G. F. Payne, X. H. Wang, and R. C. Sun

Jun 2016

Constant pH Molecular Dynamics Reveals pH-Modulated Binding of Two Small-Molecule BACE1 Inhibitors

C. R. Ellis, C. C. Tsai, X. J. Hou, and J. N. Shen

Feb 2016

Electrochemical Fabrication of Functional Gelatin-Based Bioelectronic Interface

X. H. Peng, Y. Liu, W. E. Bentley, and G. F. Payne

Jan 2016

Reverse Engineering Applied to Red Human Hair Pheomelanin Reveals Redox-Buffering as a Pro-Oxidant Mechanism

E. Kim, L. Panzella, R. Micillo, W. E. Bentley, A. Napolitano, and G. F. Payne

Dec 2015

Nano-guided cell networks as conveyors of molecular communication

J. L. Terrell, H. C. Wu, C. Y. Tsao, N. B. Barber, M. D. Servinsky, G. F. Payne, and W. E. Bentley

Oct 2015

pH-Responsive Self-Assembly of Polysaccharide through a Rugged Energy Landscape

B. H. Morrow, G. F. Payne, and J. Shen

Sep 2015

Biofabricated Nanoparticle Coating for Liver-Cell Targeting

C. Zhang, X. Qu, J. Y. Li, H. Hong, J. B. Li, J. Ren, G. F. Payne, and C. S. Liu

Jul 2015

Self-Assembly with Orthogonal-Imposed Stimuli To Impart Structure and Confer Magnetic Function To Electrodeposited Hydrogels

Y. Li, Y. Liu, T. R. Gao, B. Zhang, Y. Y. Song, J. L. Terrell, N. Barber, W. E. Bentley, I. Takeuchi, G. F. Payne, and Q. Wang

Apr 2015

Chitosan to Connect Biology to Electronics: Fabricating the Bio-Device Interface and Communicating Across This Interface

E. Kim, Y. Xiong, Y. Cheng, H. C. Wu, Y. Liu, B. H. Morrow, H. Ben-Yoav, R. Ghodssi, G. W. Rubloff, J. N. Shen, W. E. Bentley, X. W. Shi, and G. F. Payne

Dec 2014

Functionalizing Soft Matter for Molecular Communication

Y. Liu, H.-S. Wu, M. Chhuan, J. L. Terrell, C.-Y. Tsao, W. E. Bentley, and G. F. Payne

Electrical Signals Triggered Controllable Formation of Calcium-alginate Film for Wound Treatment

X. L. Liu, H. Liu, X. Qu, M. Lei, C. C. Zhang, H. Hong, G. F. Payne, and C. S. Liu

Catechol-Based Hydrogel for Chemical Information Processing

E. Kim, Z. Liu, Y. Liu, W. Bentley, and G. Payne

Electrochemical Measurement of the beta-Galactosidase Reporter from Live Cells: A Comparison to the Miller Assay

T. Tschirhart, X. Y. Y. Zhou, H. Ueda, C. Y. Tsao, E. Kim, G. F. Payne, and W. E. Bentley

Electro-molecular Assembly: Electrical Writing of Information into an Erasable Polysaccharide Medium

K. Yan, Y. Xiong, S. Wu, W. E. Bentley, H. Deng, Y. Du, G. F. Payne, and X.-W. Shi