Thin Film Biofabrication for Integrated Bio-electronics
This project integrates advances in biotechnology and microelectronics by developing generic, biologically-based thin film fabrication methods to incorporate biological function into electronics. Thin hydrogel films will be triggered to self-assemble in response to locally-imposed electrical signals. The PIs propose methods to incorporate small molecules, proteins, and cells into the hydrogel films, and will focus on understanding how fabrication conditions control the structure-property-function relations. A multilayer thin film system is proposed that will allow communication between the biology and the electronics. A collaboration with industrial partners will accelerate the incorporation of biology into materials science. The PIs also propose to integrate the activities within existing University partnerships with Food and Drug Administration and the National Institutes of Science and Technology. Because they provide the metrology and regulatory guidelines, these agencies will be integral to the speed that biologically-based materials are introduced into the marketplace. These partnerships will assist in accelerating translation and also in providing meaningful externships for students.
Publications
Parsed synthesis of pyocyanin via co-culture enables context-dependent intercellular redox communication
K. Chun, K. Stephens, S. Wang, C. Tsao, G. F. Payne, and W. E. Bentley
11/24/2021
A Redox-Based Autoinduction Strategy to Facilitate Expression of 5xCys-Tagged Proteins for Electrobiofabrication
S. Wang, C. Tsao, D. Motabar, J. Li, G. F. Payne, and W. E. Bentley
6/18/2021
Bioelectronic control of a microbial community using surface-assembled electrogenetic cells to route signals
J. L. Terrell, T. Tschirhart, J. P. Jahnke, K. Stephens, Y. Liu, H. Dong, M. M. Hurley, M. Pozo, R. McKay, C. Y. Tsao, H. Wu, G. Vora, G. F. Payne, D. N. Stratis-Cullum, and W. E. Bentley
3/29/2021
3D-Printed electrochemical sensor-integrated transwell systems
P. Ramiah Rajasekaran, A. A. Chapin, D. N. Quan, J. Herberholz, W. E. Bentley, and R. Ghodssi
10/5/2020
Electrochemical measurement of serotonin by Au-CNT electrodes fabricated on microporous cell culture membranes
A. A. Chapin, P. R. Rajasekaran, D. N. Quan, L. Hu, J. Herberholz, W. E. Bentley, and R. Ghodssi
9/7/2020
Homologous Quorum Sensing Regulatory Circuit: A Dual-Input Genetic Controller for Modulating Quorum Sensing-Mediated Protein Expression in E. coli
P. Hauk, K. Stephens, C. Virgile, E. VanArsdale, A. E. Pottash, J. S. Schardt, S. M. Jay, H. O. Sintim, and W. E. Bentley
8/21/2020
Synthetic Biology for Manipulating Quorum Sensing in Microbial Consortia
K. Stephens, and W. E. Bentley
8/1/2020
Transglutaminase-mediated assembly of multi-enzyme pathway onto TMV brush surfaces for synthesis of bacterial autoinducer-2
N. Bhokisham, Y. Liu, A. D. Brown, G. F. Payne, J. N. Culver, and W. E. Bentley
7/29/2020
Quorum Sensing Communication: Molecularly Connecting Cells, Their Neighbors, and Even Devices
S. Wang, G. F. Payne, and W. E. Bentley
6/8/2020
Mediated Electrochemistry to Mimic Biology's Oxidative Assembly of Functional Matrices
J. Li, E. Kim, K. M. Gray, C. Conrad, C. Tsao, S. P. Wang, G. Zong, G. Scarcelli, K. M. Stroka, L. Wang, W. E. Bentley, and G. F. Payne
6/2/2020
A redox-based electrogenetic CRISPR system to connect with and control biological information networks
N. Bhokisham, E. VanArsdale, K. T. Stephens, P. Hauk, G. F. Payne, and W. E. Bentley
5/15/2020
Hierarchical patterning via dynamic sacrificial printing of stimuli-responsive hydrogels
H. Wen, J. Li, G. F. Payne, Q. Feng, M. Liang, J. Chen, H. Dong, and X. Cao
4/22/2020
A Coculture Based Tyrosine-Tyrosinase Electrochemical Gene Circuit for Connecting Cellular Communication with Electronic Networks
E. VanArsdale, D. Hörnström, G. Sjöberg, I. Järbur, J. Pitzer, G. F. Payne, A. J. A. van Maris, and W. E. Bentley
3/25/2020
Redox Activities of Melanins Investigated by Electrochemical Reverse Engineering: Implications for their Roles in Oxidative Stress
E. Kim, L. Panzella, A. Napolitano, and G. F. Payne
3/1/2020
Electrical cuing of chitosan's mesoscale organization
S. Wu, K. Yan, J. Li, R. N. Huynh, C. B. Raub, J. Shen, X. Shi, and G. F. Payne
3/1/2020
Tag-Free Site-Specific BMP-2 Immobilization with Long-Acting Bioactivities via a Simple Sugar–Lectin Interaction
H. Wang, X. Qu, Z. Zhang, M. Lei, H. Tan, C. Bao, S. Lin, L. Zhu, J. Kohn, and C. Liu
2/26/2020
Melanin Produced by the Fast-Growing Marine Bacterium Vibrio natriegens through Heterologous Biosynthesis: Characterization and Application
Z. Wang, T. Tschirhart, Z. Schultzhaus, E. E. Kelly, A. Chen, E. Oh, O. Nag, E. R. Glaser, E. Kim, P. F. Lloyd, P. T. Charles, W. Li, D. Leary, J. Compton, D. A. Phillips, A. Dhinojwala, G. F. Payne, and G. J. Vora
2/18/2020
Multifunctional Conductive Hydrogel/Thermochromic Elastomer Hybrid Fibers with a Core–Shell Segmental Configuration for Wearable Strain and Temperature Sensors
J. Chen, H. Wen, G. Zhang, F. Lei, Q. Feng, Y. Liu, X. Cao, and H. Dong
1/23/2020
Redox-Channeling Polydopamine-Ferrocene (PDA-Fc) Coating To Confer Context-Dependent and Photothermal Antimicrobial Activities
J. Song, H. Liu, M. Lei, H. Tan, Z. Chen, A. Antoshin, G. F. Payne, X. Qu, and C. Liu
1/23/2020
Validation of oxidative stress assay for schizophrenia
E. Kim, Z. Keskey, M. Kang, C. Kitchen, W. E. Bentley, S. Chen, D. L. Kelly, and G. F. Payne
10/1/2019
Rapid Electroformation of Biopolymer Gels in Prescribed Shapes and Patterns: A Simpler Alternative to 3-D Printing
A. Gargava, S. Ahn, W. E. Bentley, and S. R. Raghavan
9/30/2019
Bacterial co-culture with cell signaling translator and growth controller modules for autonomously regulated culture composition
K. Stephens, M. Pozo, C. Tsao, P. Hauk, and W. E. Bentley
9/11/2019
Chip modularity enables molecular information access from organ-on-chip devices with quality control
W. Shang, C. Chen, K. Lo, G. F. Payne, and W. E. Bentley
9/1/2019
Engineering Escherichia coli for enhanced sensitivity to the autoinducer‐2 quorum sensing signal
K. Stephens, A. Zargar, M. Emamian, N. Abutaleb, E. Choi, D. N. Quan, G. Payne, and W. E. Bentley
8/10/2019
Pro- and Anti-oxidant Properties of Redox-Active Catechol-Chitosan Films
E. Kim, M. Kang, H. Liu, C. Cao, C. Liu, W. E. Bentley, X. Qu, and G. F. Payne
7/30/2019
Redox Is a Global Biodevice Information Processing Modality
E. Kim, J. Li, M. Kang, D. L. Kelly, S. Chen, A. Napolitano, L. Panzella, X. Shi, K. Yan, S. Wu, J. Shen, W. E. Bentley, and G. F. Payne
7/1/2019
Electrobiofabrication: electrically based fabrication with biologically derived materials
J. Li, S. Wu, E. Kim, K. Yan, H. Liu, C. Liu, H. Dong, X. Qu, X. Shi, J. Shen, W. E. Bentley, and G. F. Payne
4/26/2019
Redox-Based Synthetic Biology Enables Electrochemical Detection of the Herbicides Dicamba and Roundup via Rewired Escherichia coli
E. VanArsdale, C. Tsao, Y. Liu, C. Chen, G. F. Payne, and W. E. Bentley
4/16/2019
Role of polydopamine’s redox-activity on its pro-oxidant, radical-scavenging, and antimicrobial activities
H. Liu, X. Qu, H. Tan, J. Song, M. Lei, E. Kim, G. F. Payne, and C. Liu
4/1/2019
Programmable Electrofabrication of Porous Janus Films with Tunable Janus Balance for Anisotropic Cell Guidance and Tissue Regeneration
M. Lei, X. Qu, H. Liu, Y. Liu, S. Wang, S. Wu, W. E. Bentley, G. F. Payne, and C. Liu
3/11/2019
Plasmid‐encoded protein attenuates Escherichiacoli swimming velocity and cell growth, not reprogrammed regulatory functions
C. Virgile, P. Hauk, H. Wu, and W. E. Bentley
2/15/2019
Electrochemical reverse engineering to probe for drug-phenol redox interactions
Y. Wang, Y. Liu, E. Kim, B. Li, and G. F. Payne
2/1/2019
Coupling Self-Assembly Mechanisms to Fabricate Molecularly and Electrically Responsive Films
J. Li, D. Maniar, X. Qu, H. Liu, C. Tsao, E. Kim, W. E. Bentley, C. Liu, and G. F. Payne
1/7/2019
A highly conductive, pliable and foldable Cu/cellulose paper electrode enabled by controlled deposition of copper nanoparticles
Y. Yang, Q. Huang, G. F. Payne, R. Sun, and X. Wang
1/1/2019
An immune magnetic nano-assembly for specifically amplifying intercellular quorum sensing signals
Y. Guan, C. Tsao, D. N. Quan, Y. Li, L. Mei, J. Zhang, B. Zhang, Y. Liu, W. E. Bentley, G. F. Payne, and Q. Wang
12/1/2018
Electrodeposition of a magnetic and redox-active chitosan film for capturing and sensing metabolic active bacteria
Y. Li, Y. Liu, E. Kim, Y. Song, C. Tsao, Z. Teng, T. Gao, L. Mei, W. E. Bentley, G. F. Payne, and Q. Wang
9/1/2018
Catechol-chitosan redox capacitor for added amplification in electrochemical immunoanalysis
K. Yan, Y. Liu, Y. Guan, N. Bhokisham, C. Tsao, E. Kim, X. Shi, Q. Wang, W. E. Bentley, and G. F. Payne
9/1/2018
A platform of genetically engineered bacteria as vehicles for localized delivery of therapeutics: Toward applications for Crohn's disease
R. McKay, M. Ghodasra, J. Schardt, D. Quan, A. E. Pottash, W. Shang, S. M. Jay, G. F. Payne, M. W. Chang, J. C. March, and W. E. Bentley
9/1/2018
Reverse Engineering To Characterize Redox Properties: Revealing Melanin’s Redox Activity through Mediated Electrochemical Probing
M. Kang, E. Kim, Z. Temoçin, J. Li, E. Dadachova, Z. Wang, L. Panzella, A. Napolitano, W. E. Bentley, and G. F. Payne
8/17/2018
Electrical Writing onto a Dynamically Responsive Polysaccharide Medium: Patterning Structure and Function into a Reconfigurable Medium
S. Wu, K. Yan, Y. Zhao, C. Tsai, J. Shen, W. E. Bentley, Y. Chen, H. Deng, Y. Du, G. F. Payne, and X. Shi
8/14/2018
Signal processing approach to probe chemical space for discriminating redox signatures
M. Kang, E. Kim, S. Chen, W. E. Bentley, D. L. Kelly, and G. F. Payne
7/1/2018
Focusing quorum sensing signalling by nano‐magnetic assembly
Y. Guan, C. Tsao, D. N. Quan, Y. Li, L. Mei, Y. Song, B. Zhang, Y. Liu, G. F. Payne, W. E. Bentley, and Q. Wang
7/1/2018
Radical Scavenging Activities of Biomimetic Catechol-Chitosan Films
C. Cao, E. Kim, Y. Liu, M. Kang, J. Li, J. Yin, H. Liu, X. Qu, C. Liu, W. E. Bentley, and G. F. Payne
6/21/2018
Electrofabrication of functional materials: Chloramine-based antimicrobial film for infectious wound treatment
X. Qu, H. Liu, C. Zhang, Y. Lei, M. Lei, M. Xu, D. Jin, P. Li, M. Yin, G. F. Payne, and C. Liu
6/1/2018
Biofabricating Functional Soft Matter Using Protein Engineering to Enable Enzymatic Assembly
Y. Liu, H. Wu, N. Bhokisham, J. Li, K. Hong, D. N. Quan, C. Tsao, W. E. Bentley, and G. F. Payne
5/10/2018
Bio-inspired redox-cycling antimicrobial film for sustained generation of reactive oxygen species
H. Liu, X. Qu, E. Kim, M. Lei, K. Dai, X. Tan, M. Xu, J. Li, Y. Liu, X. Shi, P. Li, G. F. Payne, and C. Liu
4/1/2018
Selective assembly and functionalization of miniaturized redox capacitor inside microdevices for microbial toxin and mammalian cell cytotoxicity analyses
W. Shang, Y. Liu, E. Kim, C. Tsao, G. F. Payne, and W. E. Bentley
1/1/2018
Electrical Programming of Soft Matter: Using Temporally Varying Electrical Inputs To Spatially Control Self Assembly
K. Yan, Y. Liu, J. Zhang, S. O. Correa, W. Shang, C. Tsai, W. E. Bentley, J. Shen, G. Scarcelli, C. B. Raub, X. Shi, and G. F. Payne
12/29/2017
Spectroelectrochemical Reverse Engineering DemonstratesThat Melanin’s Redox and Radical Scavenging Activities Are Linked
E. Kim, M. Kang, T. Tschirhart, M. Malo, E. Dadachova, G. Cao, J. Yin, W. E. Bentley, Z. Wang, and G. F. Payne
11/1/2017
Connecting Biology to Electronics: Molecular Communication via Redox Modality
Y. Liu, J. Li, T. Tschirhart, J. L. Terrell, E. Kim, C. Tsao, D. L. Kelly, W. E. Bentley, and G. F. Payne
10/18/2017
Incorporating LsrK AI‐2 quorum quenching capability in a functionalized biopolymer capsule
M. K. Rhoads, P. Hauk, J. Terrell, C. Tsao, H. Oh, S. R. Raghavan, S. S. Mansy, G. F. Payne, and W. E. Bentley
10/16/2017
The Analgesic Acetaminophen and the Antipsychotic Clozapine Can Each Redox-Cycle with Melanin
Z. Temoçin, E. Kim, J. Li, L. Panzella, M. L. Alfieri, A. Napolitano, D. L. Kelly, W. E. Bentley, and G. F. Payne
10/9/2017
Reliable clinical serum analysis with reusable electrochemical sensor: Toward point-of-care measurement of the antipsychotic medication clozapine
M. Kang, E. Kim, T. E. Winkler, G. Banis, Y. Liu, C. A. Kitchen, D. L. Kelly, R. Ghodssi, and G. F. Payne
9/1/2017
Controlling localization of Escherichia coli populations using a two‐part synthetic motility circuit: An accelerator and brake
R. McKay, P. Hauk, H. Wu, A. E. Pottash, W. Shang, J. Terrell, G. F. Payne, and W. E. Bentley
8/29/2017
Toward Understanding the Environmental Control of Hydrogel Film Properties: How Salt Modulates the Flexibility of Chitosan Chains
C. Tsai, B. H. Morrow, W. Chen, G. F. Payne, and J. Shen
7/21/2017
A simple and reusable bilayer membrane-based microfluidic device for the study of gradient-mediated bacterial behaviors
W. Shang, C. Tsao, X. Luo, M. Teodoro, R. McKay, D. N. Quan, H. Wu, G. F. Payne, and W. E. Bentley
7/1/2017
Electrochemical reverse engineering: A systems-level tool to probe the redox-based molecular communication of biology
J. Li, Y. Liu, E. Kim, J. C. March, W. E. Bentley, and G. F. Payne
4/1/2017
Electrochemistry for bio-device molecular communication: The potential to characterize, analyze and actuate biological systems
Y. Liu, E. Kim, J. Li, M. Kang, W. E. Bentley, and G. F. Payne
3/1/2017
Reversible Programing of Soft Matter with Reconfigurable Mechanical Properties
H. He, X. Cao, H. Dong, T. Ma, and G. F. Payne
2/20/2017
Mathematical model of LsrR-binding and derepression inEscherichia coliK12
S. M. Graff, and W. E. Bentley
2/1/2017
Redox Probing for Chemical Information of Oxidative Stress
E. Kim, T. E. Winkler, C. Kitchen, M. Kang, G. Banis, W. E. Bentley, D. L. Kelly, R. Ghodssi, and G. F. Payne
1/12/2017
Using a Redox Modality to Connect Synthetic Biology to Electronics: Hydrogel‐Based Chemo‐Electro Signal Transduction for Molecular Communication
Y. Liu, C. Tsao, E. Kim, T. Tschirhart, J. L. Terrell, W. E. Bentley, and G. F. Payne
11/18/2016
A Facile Two-Step Enzymatic Approach for Conjugating Proteins to Polysaccharide Chitosan at an Electrode Interface
N. Bhokisham, Y. Liu, H. Pakhchanian, G. F. Payne, and W. E. Bentley
11/14/2016
Fusing Sensor Paradigms to Acquire Chemical Information: An Integrative Role for Smart Biopolymeric Hydrogels
E. Kim, Y. Liu, H. Ben‐Yoav, T. E. Winkler, K. Yan, X. Shi, J. Shen, D. L. Kelly, R. Ghodssi, W. E. Bentley, and G. F. Payne
9/12/2016
Conferring biological activity to native spider silk: A biofunctionalized protein‐based microfiber
H. Wu, D. N. Quan, C. Tsao, Y. Liu, J. L. Terrell, X. Luo, J. Yang, G. F. Payne, and W. E. Bentley
8/17/2016
Electrochemical Probing through a Redox Capacitor To Acquire Chemical Information on Biothiols
Z. Liu, Y. Liu, E. Kim, W. E. Bentley, and G. F. Payne
7/6/2016
Probing Energy and Electron Transfer Mechanisms in Fluorescence Quenching of Biomass Carbon Quantum Dots
Z. Liang, M. Kang, G. F. Payne, X. Wang, and R. Sun
6/28/2016
Paraquat–Melanin Redox-Cycling: Evidence from Electrochemical Reverse Engineering
E. Kim, W. T. Leverage, Y. Liu, L. Panzella, M. L. Alfieri, A. Napolitano, W. E. Bentley, and G. F. Payne
6/14/2016
Constant pH Molecular Dynamics Reveals pH-Modulated Binding of Two Small-Molecule BACE1 Inhibitors
C. R. Ellis, C. Tsai, X. Hou, and J. Shen
2/25/2016
Electrochemical Fabrication of Functional Gelatin-Based Bioelectronic Interface
X. Peng, Y. Liu, W. E. Bentley, and G. F. Payne
1/21/2016
Reverse Engineering Applied to Red Human Hair Pheomelanin Reveals Redox-Buffering as a Pro-Oxidant Mechanism
E. Kim, L. Panzella, R. Micillo, W. E. Bentley, A. Napolitano, and G. F. Payne
12/16/2015
Nano-guided cell networks as conveyors of molecular communication
J. L. Terrell, H. Wu, C. Tsao, N. B. Barber, M. D. Servinsky, G. F. Payne, and W. E. Bentley
10/12/2015
pH-Responsive Self-Assembly of Polysaccharide through a Rugged Energy Landscape
B. H. Morrow, G. F. Payne, and J. Shen
9/30/2015
Biofabricated Nanoparticle Coating for Liver‐Cell Targeting
C. Zhang, X. Qu, J. Li, H. Hong, J. Li, J. Ren, G. F. Payne, and C. Liu
7/2/2015
Self-Assembly with Orthogonal-Imposed Stimuli To Impart Structure and Confer Magnetic Function To Electrodeposited Hydrogels
Y. Li, Y. Liu, T. Gao, B. Zhang, Y. Song, J. L. Terrell, N. Barber, W. E. Bentley, I. Takeuchi, G. F. Payne, and Q. Wang
5/11/2015
Chitosan to Connect Biology to Electronics: Fabricating the Bio-Device Interface and Communicating Across This Interface
E. Kim, Y. Xiong, Y. Cheng, H. Wu, Y. Liu, B. Morrow, H. Ben-Yoav, R. Ghodssi, G. Rubloff, J. Shen, W. Bentley, X. Shi, and G. Payne
12/24/2014
Electrical Signals Triggered Controllable Formation of Calcium-alginate Film for Wound Treatment
Electro-molecular Assembly: Electrical Writing of Information into an Erasable Polysaccharide Medium
Electrochemical Measurement of the beta-Galactosidase Reporter from Live Cells: A Comparison to the Miller Assay
Catechol-Based Hydrogel for Chemical Information Processing
Functionalizing Soft Matter for Molecular Communication
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Research Highlights
Elucidating Salt Effects on Chitosan Dynamics
Gregory Payne and Jana Shen
7/21/2017
Translating Bioelectrochemical Engineering
Gregory Payne and William Bentley
2/3/2023
A New Paradigm for Accessing Chemical Information
Gregory Payne and William Bentley
2/3/2023
Industrial Collaboration:pH-responsive Inhibitors
Jana Shen
2/23/2016
Reconfiguring Hydrogels by Switching Crosslinks
Gregory Payne
2/20/2017
Enlisting Synbio for Molecular Communication
Gregory Payne and William Bentley
11/18/2016
Reverse Engineering of Materials Properties
Gregory Payne and William Bentley
10/15/2017
Extending Reverse Engineering to Biology
Gregory Payne and William Bentley
6/1/2016
Attracting College and High School Students to STEM
Jana Shen
6/23/2017
Scientists as Ambassadors
Gregory Payne
2/20/2017
View All Highlights